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- flim_fret_calculation_for_multi_exponential_donors @howto
- wer center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... FRET distance histogram.\\ **Lower center/left:** TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... on is deducted from the rising edge of the images TCSPC histogram. On the left of this graph, the fitting
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- fitting_script_Image_15.png }} **Response:** The TCSPC fitting window is opened and the TCSPC histograms of both files are loaded. The TCSPC histogram from the file "ATTO655_diff_FLCS-patter... etime measurement of the dye Cy5 in water. In the TCSPC histogram it can be clearly seen that the lifetim
- tcspc_external_markers @support
- {{tag>TCSPC scanner markers TTTR Harps PH300 HH400 TH260 SymPhoTime64}} ~~TOC~~ ====== TCSPC modules and External Markers====== Here a general... rkers from a controller of scanning module to the TCSPC module. The markers are incorporated into the raw data according to the principles of [[http://tcspc.com/doku.php/glossary:tttr|Time Tagged Time Resol
- software
- are the maintainer of a software that deals with TCSPC data please edit this page and add your software ... me Correlated Single Photon Counting Instruments (TCSPC devices). It harnesses the advantages of C++ for ... een the high-speed capabilities of your PicoQuant TCSPC device and the ease of use and versatility of Pyt... is of raw Time-Correlated Single Photon Counting (TCSPC) Fluorescence Lifetime Imaging Microscopy (FLIM)
- lifetime-fitting_using_the_flim_script @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- roi_fitting_using_the_flim_script @howto
- wer center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... on is deducted from the rising edge of the images TCSPC histogram. * To enhance the lifetime contrast ... he 0 value at the rising edge of the decay in the TCSPC window. As a mean arrival time is calculated, it ... e randomly detected before the laser pulse in the TCSPC window.\\ Reasonable limits can either be found b
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime_fitting_using_the_flim_analysis @howto
- wer center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... struction is deducted from the rising edge of the TCSPC histogram. * To enhance the lifetime contrast ... he 0 value at the rising edge of the decay in the TCSPC window. As a mean arrival time is calculated, it ... e randomly detected before the laser pulse in the TCSPC window.\\ Reasonable limits can either be found b
- separation_of_2_species_with @howto
- fluorescence lifetime measurements via [[glossary:tcspc|TCSPC]], this script can only be applied, if at least o... nents are available, it is possible to load these TCSPC histograms as patterns for the filter definition.... ttern Matching" approach. * If the individual TCSPC histograms are not available, or a dye is analyze... f the different parameters. - **Upper center:** TCSPC histogram of the imported patterns. As we haven't
- update @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- symphotime_tips_and_tricks @howto
- opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on the type of an... Time-Trace === * Max. Counts * Av. Counts === TCSPC === * Max. Counts (number of photons in the peak TCSPC-channel) You can also add additional information... ts can be set (e.g. 0 as minimum lifetime) in the TCSPC fitting panel * the fast lifetime in an ideal s
- how_to_work_with_the_instrument_response_function_irf @howto
- 57fcfa684daef86c23]].\\ 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; {{ howto:tcspc_decay_gui.png?400 |}} Response: you see this kind of curve: {{ howt... as these decays contain the noise of the complete TCSPC-window, the end of the fitting curve might show n... ype of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- detected, is randomly spread over the [[glossary:TCSPC]] window, the mean photon arrival time of the dar... is usually very long (up to 1/2 of the [[glossary:TCSPC]] window), which makes the color scale loaded by ... pplied to the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\ - **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green
- pile-up_effect @glossary
- {{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the e... gh photon count rates due to the dead time of the TCSPC devices. Most single photon counting detectors and [[TCSPC]] electronics have a [[dead time]]. After registe... for the next photon to detect. The dead time for TCSPC devices used for the measurement of fluorescence