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syntax @wiki
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ormatted. You can use <sub>subscript</sub> and <sup>superscript</sup>, too. You can use <sub>subscript</sub> and <sup>superscript</sup>, too. You can mark something as <del>deleted</d... ]] Generic Syntax Highlighter -- so any language supported by GeSHi is supported. The syntax uses the same code and file blo... v dos dot d ecmascript eiffel email epc e erlang euphoria ezt f1 falcon fo fortran freebasic freeswitc
supported_mt200_pc_configuration_for_symphotime_32 @software
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sted PC from PicoQuant. While we try our best to support custom PC setups we cannot guarantee that all the components will... ivers for Network, Mainboard etc. * Run windows update to fetch all the latest updates * Install the Peak CAN PCI card * Start ... ardware settings. ===== TCSPC Modules ===== * Supported: TimeHarp 200, all versions PicoHarp 300, up to version HydraHarp 400, up to version 1.2 * NOT SUPPORTED: TimeHarp 260, HydraHarp 2.0 and higher
configuring_symphotime64_after_installation @support
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ce driver and press on "Connect". * You can set up the Laser Heads by pressing "Setup Heads" and a new window will pop up: {{ :support:laser_settings_setup_head.png?300 |}} You can set: * the name of the laser head... Do not forget to press "Save Head" after setting up each Laser Head" </WRAP> ====3. For LSM upgrade kits==== Go to the SymPhoTIme Manual page 6 under "
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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es through the detection volume of a confocal set-up. Since then a series of studies have been perfor... living cells, including for example diffusion -coupled interactions like ligand binding, oligomerizat... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fluorescent molecules in a confocal setup the origin of the intensity fluctuations, will be
software
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isting. Alternatively you can also use [[https://support.picoquant.com]] to contact PicoQuant support to submit your software project. ==== Data ... orescence Spectroscopy and Imaging by research group Seidel, HHU]]: A collection of independent sotftw... rfrom the analysis workflow put forward by the group.Please note that a trial version is available. ... -for-Single-Molecule-Spectroscopy|Researchgate group Open Computational Tools for Single-Molecule Spec
supported_mt200_pc_configuration_for_symphotime_64 @software
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sted PC from PicoQuant. While we try our best to support custom PC setups we cannot guarantee that all the components will work together. =====Supported OS ===== * Win10: YES * Win8: YES * W... : YES * Win7 32bit: NO * WinXP: NO =====Supported Hardware (for MicroTime200):===== ====Harp... s)==== ===PicoHarp 300=== * For imaging, only supported with Router PHR800 * Supported for point
flim_fret_calculation_for_multi_exponential_donors @howto
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pplication note [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]]. Th... The window contains five different regions:\\ **Upper left:** Imaging analysis options.\\ **Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
avoid_pile_up_effect_in_flim_measurements @howto
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easurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile-up effect]] the measured count rate should not exceed a certain li... or most FLIM measurements this relative weak pile up effect is acceptable. If even higher precision ... ate of the laser. ===== Measurement of the Pile Up Limit ===== * For setting the right laser int
lifetime-fitting_using_the_flim_script @howto
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me and contains e.g. background photons.\\ * An updated lifetime frequency histogram on the upper right. The individual lifetime components are
symphotime_tips_and_tricks @howto
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post-acquisition analysis is performed - speeds up the processing for additional analysis. - This ... times) * Frames (especially important for LSM upgrade kits where in general several frames are rec... with the MT200 this is done automatically, in LSM upgrade systems, which are not remotely controlled b... d "Pattern Matching" the feature is made availble upon clicking on the **"Resolution estimation"** tab
separation_of_2_species_with @howto
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s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ separation_of_2_species_with_Image_8.png?600 ... ut the meaning of the different parameters. - **Upper center:** TCSPC histogram of the imported patt... n yet, this graph doesn't contain any data. - **Upper right:** TCPSC curve of the measured data. Low
how_to_reinstall_symphotime
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he installation DVDs any more, contact PicoQuant support (support@picoquant.com) for the download links for you... filename of the existing file to have it as a backup (for example as "settings_default.pfs"). - Re... oTime with these user settings. ====== I want to update my SymPhoTime 64 to the latest version. What ... need to do? ====== {{tag> faq SPT SPT64 MT200}} Updates for SymPhoTime 64 are free. You can always g
pile-up_effect @glossary
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tion pulse is negligible. * [[howto:avoid_pile_up_effect_in_flim_measurements|How to Avoid the Pile-up Effect in FLIM Measurements]] ===== Example of pile up effect ===== We have performed an example measur... ional short component lifetime could still not be detected {{ :glossary:pile-up_example.png?600 |}}
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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o the tutorial [[Calculate FCCS Trace with the Grouped FCS Script]]. **Note:** This way you can compa... s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ using_the_flcs_script_for_spectral_crosstalk_... ut the meaning of the different parameters. - **Upper center:** The graph for the imported patterns.
lifetime_fitting_using_the_flim_analysis @howto
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e and contains e.g. background photons. * An updated lifetime frequency histogram on the upper right. The fixed individual lifetime component
asymptotic_standard_errors @glossary
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start
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microtime @products
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tcspc_external_markers @support
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t3-mode @glossary
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intensity_time_trace_analysis @howto
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roi_fitting_using_the_flim_script @howto
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update @howto
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calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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calculate_ratiometric_fret-images @howto
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welcome @wiki
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using_the_anisotropy_image_script @howto
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measurement_hardware_instrumentation
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lsm710
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t2-mode @glossary
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differential_count_rate @glossary
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antibunching_measurements @howto
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2ffcs @howto
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diamond_nv_centers @howto
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align_beam_backreflection @howto
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supported_th260_pc
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dokuwiki @wiki
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pycorrfit @software
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support_plane_analysis @glossary
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laser_intensity_response @faq
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symphotime64 @software
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chi_square_management @glossary
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using_the_antibunching_script @howto
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registering_new_scripts @howto
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tttr @glossary
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contributing @contributing
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exchange_dichroic_mt200 @howto
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reconvolution_fit @howto
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dead_time @glossary
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data_file_import @howto
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pattern_matching @howto
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novaflim_calculated_irf @faq
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lifetime_component_decomposition_phasor_plot_analysis
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community_news @general
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hybrid_pmt @glossary
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determination_of_the_focal_width_with_the_focal @howto
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bootstrap @glossary
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easytau @software
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howto @howto
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imprint
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create_time_gated_image
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symphotime @software
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ax_flim_examples @howto:ax_flim_examples
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applications
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symphotime_32 @software
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flimfit @software
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aotf @glossary
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bifl @glossary
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cfd @glossary
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