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- avoid_pile_up_effect_in_flim_measurements
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Fulltext results:
- avoid_pile_up_effect_in_flim_measurements @howto
- easurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile-up effect]] the measured count rate should not exceed a certain li... or most FLIM measurements this relative weak pile up effect is acceptable. If even higher precision ... ate of the laser. ===== Measurement of the Pile Up Limit ===== * For setting the right laser int
- configuring_symphotime64_after_installation @support
- ce driver and press on "Connect". * You can set up the Laser Heads by pressing "Setup Heads" and a new window will pop up: {{ :support:laser_settings_setup_head.png?300 |}} You can set: * t... Do not forget to press "Save Head" after setting up each Laser Head" </WRAP> ====3. For LSM upgrade ... picoquant.com/dl_software/SPT-RC/SymPhoTime_RC_Setup.zip| SymPhoTime 64 Remote Controller ]] ====4. F
- pile-up_effect @glossary
- tion pulse is negligible. * [[howto:avoid_pile_up_effect_in_flim_measurements|How to Avoid the Pile-up Effect in FLIM Measurements]] ===== Example of pile up effect ===== We have performed an example measur... ional short component lifetime could still not be detected {{ :glossary:pile-up_example.png?600 |}}
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- es through the detection volume of a confocal set-up. Since then a series of studies have been perfor... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fluorescent molecules in a confocal setup the origin of the intensity fluctuations, will be... in FCS studies. Assume that we use the same set-up to measure the diffusion of three globular fluo
- syntax @wiki
- ormatted. You can use <sub>subscript</sub> and <sup>superscript</sup>, too. You can use <sub>subscript</sub> and <sup>superscript</sup>, too. You can mark something as <del>deleted</d... at these only make sense in a homogeneous user group like a corporate [[wp>Intranet]]. Windows Shar... parentheses. ===== Sectioning ===== You can use up to five different levels of headlines to structur
- old_dongles_drivers_for_fluofit_symphotime_32 @faq:old_dongles_drivers_for_fluofit_symphotime_32
- ot your system. Install Sentinel_LDK_Run-time_setup [[https://supportportal.thalesgroup.com/csm?sys_kb_id=61fb0ee1dbd2e78cfe0aff3dbf9619a... ysparm_article=KB0018320|Sentinel_LDK_Run-time_setup]] (available on the Sentinel webpage; exact locat... old to run properly on Windows 10/11, you can set up a Windows 7 Virtual Machine (VM) and use the last... packages). Our team successfully tested this setup using VMware Workstation Player 15.5.1, where bot
- asymptotic_standard_errors @glossary
- parameter axis. We then calculate the reduced X<sup>2</sup> for this new paramter set. By iterating this procedure we get X<sup>2</sup> as a function of the parameter of interes... ersection points of this function with a given X<sup>2</sup> confidence limit define the boundaries of the co
- t3-mode @glossary
- d to a sync signal. As far as the experimental setup is concerned, this is similar to histogramming mo... . The main objective is to allow high sync rates (up to 84 MHz) which could not be handled in T2 Mode.... nted by four bits in each TTTR record, that allow up to four routing channels to be distinguished. How... ]] (First In First Out) buffer capable of holding up to 256 k event records. The FIFO input is fast en
- supported_mt200_pc_configuration_for_symphotime_32 @software
- pported: TimeHarp 200, all versions PicoHarp 300, up to version HydraHarp 400, up to version 1.2 * NOT SUPPORTED: TimeHarp 260, H
- differential_count_rate @glossary
- / = 100 000 counts ⁄ (10 000 000 pulses · 150·10<sup>-12</sup> second) ≅ 66 666 667 cps This is still just an
- flim_fret_calculation_for_multi_exponential_donors @howto
- time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ates have been significantly higher than the pile up limit, or in cases, where the repetition rate has... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ET efficiency around 0 is expected and also shows up in the histogram. * On the lower right, the
- separation_of_2_species_with @howto
- esponse:** The FLCS pattern matching windows pops up. {{ separation_of_2_species_with_Image_8.png?600 ... Image_9.png }} **Response:** Another window pops up containing the measurement files from the workspa... _Image_13.png }} **Response:** A new window pops up containing the measurement files from the workspa... g }} **Response:** The TCSPC fitting window pops up. {{ separation_of_2_species_with_Image_28.png?600
- microtime @products
- 75 nm to 810 nm * Multiple detector options for up to four detection channels * XY-scanning piezo ... imaging * Wide range scanner with a scan range up to several cm See the picoquant.com webpage for... r multicolor excitation from 375 nm to 900 nm * Up to 4 truly parallel detection channels using appl
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- ript_Image_17.png }} **Response:** A window pops up asking for a file name and a place to store the u... your profile there. **Response:** A window pops up with the message that the software needs to be re... _Image_21.png?600 }} **Response:** A window pops up with the message that the software needs to be re
- how_to_reinstall_symphotime
- stay as it is. However it is a good idea to back-up the files mentioned below. **Please note: The Fr... on" - Find the settings.pfs file and make a backup copy. (This files contains all the basic hardware... filename of the existing file to have it as a backup (for example as "settings_default.pfs"). - Re