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tion with PicoQuant Time Correlated Single Photon Counting Instruments (TCSPC devices). It harnesses the adv... lot analysis of raw Time-Correlated Single Photon Counting (TCSPC) Fluorescence Lifetime Imaging Microscopy ... upport the use of a Time-Correlated Single Photon Counting system for application to Positron Annihilation L... package to analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence lifetime a
differential_count_rate @glossary
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sic principles of TCSPC in order to avoid various counting losses.(E.g. to avoid the [[pile-up effect]]). Ho... several orders of magnitude. This causes not only counting losses (=> invalid IRF shape) but also an increas... and Tahei Tahara, Note: Simple calibration of the counting-rate dependence of the timing shift of single pho
intensity_time_trace_analysis @howto
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for the two channels.\\ **On the right**, photon counting histograms (PCH) are plotted, indicating the freq... ignals in Images ==== **Note:** PicoQuant photon counting boards can register up to four external marker si
antibunching_measurements @howto
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ore S., Laurence T.A., Hollars C. W., Huser T.: //Counting constituents in molecular complexes by fluorescen... : //Experimental approach to extend the range for counting fluorescent molecules based on photon-antibunchin
using_the_antibunching_script @howto
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complete trace above marked in green. The photon counting histogram on the right displays the frequencies o... detectors arrive a different times at the photon counting TCSPC unit. Consequently, a systematic shift of t
dead_time @glossary
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ary:Pile-up Effect]]. Note that not only TCSPC devices but also photon counting devices exhibit dead times.
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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ectroscopy by using time-correlated single photon counting. \\ Triffo et al, 2012, "Monitoring Lipid Anchor
picoharp_300 @products
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use, plug and play Time-Correlated Single Photon Counting (TCSPC) system. Manufactured and sold by PicoQuan
hydraharp_400 @products
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Analyzer (TIA) and Time-Correlated Single Photon Counting (TCSPC) system with scalability for multiple chan
microtime @products
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y:Hybrid PMT]]s * Time-Correlated-Single Photon Counting ([[glossary:TCSPC]]) and [[glossary:TTTR]] mode f
t3-mode @glossary
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chosen:\\ The time tag is now obtained by simply counting sync pulses. From the T3 Mode event records it is
lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
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ector pulses will overlap and merge into one. The counting and timing system can then no longer resolve the
hybrid_pmt @glossary
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ttps://www.picoquant.com/products/category/photon-counting-detectors/pma-hybrid-series-hybrid-photomultiplie
spad @glossary
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=== SPAD ====== SPAD means **S**ingle **P**hoton counting **A**valanche photo-**D**iode. It stands commonly
mcp @glossary
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ufficiently small to perform time-resolved photon counting on a sub-nanosecond- scale, usually outperforming
basics
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pile-up_effect @glossary
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calculate_fccs_trace_with_the_grouped_fcs_script @howto
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tcspc_electronics @products
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calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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flim_fret_calculation_for_multi_exponential_donors @howto
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using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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separation_of_2_species_with @howto
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calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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