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- symphotime_tips_and_tricks
- ace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^ |''.ptu''... ==== * double click on any ''.ptu'' (raw data) file opens a file viewer, in which you find information about the file type and acquisition settings <imgcaption image1... contain a comment which can be displayed via the file menu: **File → Show Comment**. {{show_comment.png|}} Typica... Images can be drawn in 3 ways (for this, open the file ''FLIM_3_expon.pqres'', associated to the raw data file ''DaisyPollen_cells_FLIM.ptu'') * toggling betw
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- et_script_Image_14.png }} **Response:** A result file (''PIE_FRET_TimeTrace_1.pqres'') is stored under the raw data file (''Cy3+Cy5_diff_PIE-FRET.ptu''). {{ calculate_rat... mage_15.png }} * Later, clicking on the result file reopens the file in the same way as it was stored. * Now the nec... nse:** A window opens to select the user settings file. Select the recently stored file. {{ calculate_ratiometric_single_pair_fret_distri... * If the script is applied the next time to any file, the newly defined parameters will be used. **No
- lifetime_fitting_using_the_tcpsc_fitting_script
- ing_script_Image_8.png }} **Response:** A result file (''TCSPC_Fitting.pqres'') is stored under the raw data file (''ATTO655_diff_FLCS-pattern.ptu''). {{ lifetime_... Image_9.png }} * Later, clicking on the result file reopens the file in the same way as it was stored. * Now all nec... 2 }} **Response:** A window opens and asks for a file name to store the exported result file. Select e.g. the name ''decay.dat''. {{ lifetime_... marked in green, indicating that it is the active file. Under decay data all files are listed, the active file is always highlighted in green. **Note:** The fi
- calculate_ratiometric_single_pair_fret_distributions
- ributions_Image_13.png }} **Response:** A result file (''FRET_TimeTrace_1.pqres'') is stored under the raw data file (''Cy3+Cy5_diff_PIE-FRET.ptu''). {{ calculate_rat... mage_14.png }} * Later, clicking on the result file reopens the file in the same way as it was stored. * Now the nec... nse:** A window opens to select the user settings file. Select the recently stored file. {{ calculate_ratiometric_single_pair_fret_distri... * If the script is applied the next time to any file, the newly defined parameters will be used. **No
- roi_fitting_using_the_flim_script
- mage.\\ **1x1 bitmap** exports the image as a bmp file with one pixel of the image being exported as one pixel in the image file. In the other bitmap export options, the image is... 00 }} **Response:** A window opens, asking for a file name to store the fitting values. The data are stored as a ''.dat'' file. {{ roi_fitting_using_the_flim_script_Image_26.pn... Image_28.png?241 }} **Response:**\\ * A result file (''FLIM.pqres'') is stored under the raw data file (''DaisyPollen_FLIM.ptu''). {{ roi_fitting_using_... mage_29.png }} * Later, clicking on the result file reopens the file in the same way as it was stored. **Note:**\\ Th
- data_file_import
- ort.png | Selects the file to be imported }} The file selected above is a sample data file, ''Decay_Coumarin_6.phu'' installed during the in... ''.phu'' files. This is no problem, but then the file import procedure has to be repeated for each file and the imported curves will end up in dedicated ... now their assignment yet. \\ \\ ==== Multiple File Import ==== Importing multiple data files is ea... 2_fileimport2.png |}} \\ \\ Each input ''.phu'' file has got a dedicated ''.etc'' data container: {{ :
- separation_of_2_species_with
- ===== Step-by-Step Tutorial ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... _2_species_with_Image_1.png }} * Highlight the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu'' by a single mou... **Response:** The FLCS script is applied to the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu''. Thereby, a new
- flim_fret_calculation_for_multi_exponential_donors
- esult_bearb.png?400 |}} **Response:**\\ A result file (''FLIM.pqres'') is stored under the raw data file (''CENP-labelled_cells_for_FRET.ptu''). {{ :howto... _donors_Image_44.png }} **Response:**\\ A result file is saved and linked to the raw data file. {{ :howto:flim-fret-multiexpd_pqres_2.png?400 |}
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
- oval_via_flccs_Image_9.png?336 }} * Select the file ''IBA488_IBA547_unlinked_mix.ptu'' (the same file again) and click "OK". {{ using_the_flcs_script_f... **Response:** * The TCSPC histogram for this file appears in the "Pattern" graph in the upper cente... visible, as the pattern was created from the same file, which makes the fitting perfect. * Fitting v... the fluorescence decay is named according to the file (it corresponds to the filter for the pulsed exci
- using_the_anisotropy_image_script
- ge_script_Image_16.png }} **Response:** A result file (''AnisotropyImage_1.pqres'') is stored under the raw data file (''Cy5_immo_FLIM_Po-Imaging.ptu''). {{ using_the_... mage_17.png }} * Later, clicking on the result file reopens the file in the same way as it was stored. \\ ==== Stor... nse:** A window opens to select the user settings file. Select the recently generated file. {{ using_the_anisotropy_image_script_Image_23.pn... now the "Anisotropy Imaging" script again on the file, the user defined G-factor and detector assignmen
- calculate_fccs_trace_with_the_grouped_fcs_script
- '. **Response:**\\ The correlation curves of this file are calculated. The cross-correlation amplitude of this file is ~20% of the autocorrelations. {{ calculate_fc
- visualizing_dynamics_using_the_multiframe-flim_script
- image. - 1x1 bitmap exports the image as a bmp file with one pixel of the image being exported as one pixel in the image file. In the other bitmap export options, the image is... Save Results".\\ **Response:**\\ A result file is generated which is assigned to the raw data file. Double click on this ''.pqres''-file opens it in the same way as it was stored.
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- mage.\\ **1x1 bitmap** exports the image as a bmp file with one pixel of the image being exported as one pixel in the image file. In the other bitmap export options, the image is... _dynamics_Image_18.png }} **Response:** A result file is generated which is assigned to the raw data file. Double click on this ''.pqres'' file opens it in the same way as it was stored. {{ visualizing_dynamic
- intensity_time_trace_analysis
- setups. * In the sample workspace, select the file ''GUVs.ptu'' and apply the time trace script to that file. When the intensity time trace window opens, clic... . * In this way, the marker assignment for this file has been checked and the window can be closed. As we are not interested in the data itself, there is no need to store this file.
- lifetime-fitting_using_the_flim_script
- "Import" IRF button and select the corresponding file from the file list (make sure that the IRF is taken from the de... click "Save Result".\\ **Response:**\\ A result file "FLIM.pqres" is stored under the raw data file ''Kidney_Cell_FLIM.ptu''.\\ * Later, clicking on the result file reopens the file in the same way as it was stored.