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- privacy_policy
- tes System eine Reihe von allgemeinen Daten und Informationen. Diese allgemeinen Daten und Informationen werden in den Logfiles des Servers gespeiche... den Systems und (8) sonstige ähnliche Daten und Informationen, die der Gefahrenabwehr im Falle von Angriffen auf unsere informationstechnologischen Systeme dienen. Bei der Nutzu... erangriffes die zur Strafverfolgung notwendigen Informationen bereitzustellen. Diese anonym erhobenen Daten und Informationen werden durch die PicoQuant GmbH daher einers... n personenbezogenen Daten gespeichert. Logfile-Informationen werden aus Sicherheitsgründen (z.B. zur Aufk
- syntax @wiki
- wiki:dokuwiki-128.png}}]] Please note: The image formatting is the only formatting syntax accepted in link names. The whole [[#... y not all browsers understand all video and audio formats. To mitigate the problem, you can upload your file in different formats for maximum browser compatibility. For example ... l separator before a cell which decides about the formatting: | ^ Heading 1 ^ H... e: Vertical alignment is not supported. ===== No Formatting ===== If you need to display text exactly li
- symphotime_tips_and_tricks @howto
- t in the FLIM-script, can be exported in an ASCII-format as well as in a bitmap format. If the graphs consists on different cells, e.g.
- software
- rpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model func... //github.com/PicoQuant/PicoQuant-Time-Tagged-File-Format-Demos| Demo Code for reading PicoQuants PTU File format]] * [[https://github.com/Brinkslab/ptu_flim_to... [[https://www.openmicroscopy.org/site/support/bio-formats5/formats/picoquant-bin.html]] * **ImageJ data reader... Homepage]] and [[http://dx.doi.org/10.1093/bioinformatics/btv687 | paper ]] * [[https://github.com
- how_can_i_analyze_my_data_using_non-picoquant_software @faq
- so find a description of the headers and the file format(s): https://github.com/PicoQuant/PicoQuant-Time-Tagged-File-Format-Demos/tree/master/doc These scripts are meant as... rent TCSPC devices ("Harps"), will have different formats. Some open source software packages are designed for only one specific device file format.
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- gle FRET pair can be analyzed, e.g. to observe conformational changes.\\ To record such traces, usually si... h was used to excite the Cy5 dye directly. This information is used to distinguish molecules with and with... lecules as in this example file. For observing conformational fluctuations of a single immobilized molecul... 1. The other two peaks are due to twodifferent conformations of the RNA oligonucleotide measured in this s
- pycorrfit @software
- rpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model func... T. PyCorrFit - generic data evaluation for fluorescence correlation spectroscopy. Bioinformatics 2014. [[http://dx.doi.org/10.1093/bioinformatics/btu328]]
- calculate_ratiometric_single_pair_fret_distributions @howto
- gle FRET pair can be analyzed, e.g. to observe conformational changes.\\ To record such traces, usually si... h was used to excite the Cy5 dye directly. This information is not used in this script (in fact, for this ... lecules as in this example file. For observing conformational fluctuations of a single immobilized molecul... . The other two peaks are due to two different conformations of this RNA oligonucleotide measured in this
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- rts the image as an ASCII array using a text-file format. Thereby one array encodes the intensity values, ... mage series as a series of binary files. The data format of these files is described on the SymPhoTime Online help. The binary files contain the photon information of each TCSPC-channel and can be imported e.g.... icroTime system, or you transfer the image size information automatically via a handshake software, as in
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- relation function ===== In general all relevant information is conveyed by the normalised autocorrelation ... or because of the internal flexibility of the conformation of the biomolecule the quencher comes closer t... luorescence one can infer the timescale of the conformational changes of the molecule under study. \\ {{:general:conformational_change_quencher.png?200|}} ==== A bit more
- phasor_plot_structure_separation @howto:phasor_plot_structure_separation
- iological systems. **Process** * **Software information:** NIS-Elements version 6.10.01 with PicoQuant... {::nis_phasor_publish.mp4?1000|}} * **Sample information:** * Daisy pollen --- * **Software information:** NIS-Elements version 5.42.06 with PicoQuant... sition_in_phasor_plot.mp4?1000|}} * **Sample information:** * Glial Fibrillary Acidic Protein (GF
- lifetime_component_decomposition_phasor_plot_analysis
- iological systems. **Process** * **Software information:** NIS-Elements version 6.10.01 with PicoQuant... {::nis_phasor_publish.mp4?1000|}} * **Sample information:** * Daisy pollen --- * **Software information:** NIS-Elements version 5.42.06 with PicoQuant... sition_in_phasor_plot.mp4?1000|}} * **Sample information:** * Glial Fibrillary Acidic Protein (GF
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- et al., 2005, Biophys. J. 89, 4029-4042)).\\ **Conformational:** Use this model if your dye is undergoing conformational changes when diffusing through the confocal
- calculate_ratiometric_fret-images @howto
- youtube>rOas2iDwUzY?large }} ===== Background Information ===== [[glossary:fret|FRET]] is a method to determine molecular interactions or conformational changes based on a suited donor/acceptor pai... " and "7" for "Cross Talk". **Note:** For more information about the equation used for image calculation,
- imprint
- Quant GmbH logo are registered trademarks. This information could contain technical inaccuracies or typographical errors, and information will be changed, updated and deleted without n