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fluorescence_correlation_spectroscopy-_a_short_introduction
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e is. One can get FCS curve with high quality air objectives. The NA or air objectives is less than 1, resulting in an elongated volume... er practical issue is the working distance of the objective in combination with the refractive index mismatch... get a reasonable FCS curve?** \\ Water immersion objectives (for example Olympus UPLSAPO 60x/1.2w) have a la... er than 200 µm . On the other hand, oil immersion objectives (for example Olympus UPLSAPO 100x/oil) have work