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measuring_quantum_yield
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holder has two indexed positions. When in the IN position, the excitation beam will directly hit the sample, when in the OUT position the sample will only be illuminated indirectly (b... oving the sample holder make sure it is in the IN-Position. * Insert the blank sample into the sample hold... egrating sphere put the sample holder into the IN-position. * Select either IN or OUT position. **Note:** The position should be the same as for the sample measure
intensity_time_trace_analysis
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return from the end of the line to the line start position is done with highest possible speed. In contrast,
diamond_nv_centers
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w NV-centers (see figure 5), *select one, and position the focus on the NV-center. *use the oscillos
2ffcs
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the time trace oscilloscope optimize the pinhole position **with** Nomarski Prism and **both** lasers.
burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
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n depends on the diffusion coefficient, the entry position of the molecule, and the spatial weighting impose
using_the_antibunching_script
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lly moves the red and yellow curve to the correct position. Press "Calculate" again to recalculate the corre
align_beam_backreflection
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ch quadrant. To reach this, you have to precisely position the beam to enter at the center of the objective
select_the_correct_pinhole_size
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ger than the image of the [[wp>Airy Disc]] at the position of the pinhole: $$d_{Airy} = (1.22 * lambda / N.
how_to_work_with_the_instrument_response_function_irf
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power of the laser changes the shape and temporal position of the laser pulse. ((You can use the driver sett
how_to_measure_the_instrument_response_function_irf
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or upon changes of the excitation wavelength, the position of the IRF can shift slightly, but this is accoun
pattern_matching
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composing the overall decay, it performs the decomposition on each pixel. The linear decompositions of the p
phasor_plot_structure_separation @howto:phasor_plot_structure_separation
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full integration {{:lifetime_component_decomposition_in_phasor_plot.mp4?1000|}} * **Sample informat