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- flim_fret_calculation_for_multi_exponential_donors
- wer center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... FRET distance histogram.\\ **Lower center/left:** TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... on is deducted from the rising edge of the images TCSPC histogram. On the left of this graph, the fitting
- lifetime_fitting_using_the_tcpsc_fitting_script
- fitting_script_Image_15.png }} **Response:** The TCSPC fitting window is opened and the TCSPC histograms of both files are loaded. The TCSPC histogram from the file "ATTO655_diff_FLCS-patter... etime measurement of the dye Cy5 in water. In the TCSPC histogram it can be clearly seen that the lifetim
- lifetime-fitting_using_the_flim_script
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- roi_fitting_using_the_flim_script
- wer center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... on is deducted from the rising edge of the images TCSPC histogram. * To enhance the lifetime contrast ... he 0 value at the rising edge of the decay in the TCSPC window. As a mean arrival time is calculated, it ... e randomly detected before the laser pulse in the TCSPC window.\\ Reasonable limits can either be found b
- visualizing_dynamics_using_the_multiframe-flim_script
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime_fitting_using_the_flim_analysis
- wer center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... struction is deducted from the rising edge of the TCSPC histogram. * To enhance the lifetime contrast ... he 0 value at the rising edge of the decay in the TCSPC window. As a mean arrival time is calculated, it ... e randomly detected before the laser pulse in the TCSPC window.\\ Reasonable limits can either be found b
- separation_of_2_species_with
- fluorescence lifetime measurements via [[glossary:tcspc|TCSPC]], this script can only be applied, if at least o... nents are available, it is possible to load these TCSPC histograms as patterns for the filter definition.... ttern Matching" approach. * If the individual TCSPC histograms are not available, or a dye is analyze... f the different parameters. - **Upper center:** TCSPC histogram of the imported patterns. As we haven't
- update
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSP
- symphotime_tips_and_tricks
- opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on the type of an... Time-Trace === * Max. Counts * Av. Counts === TCSPC === * Max. Counts (number of photons in the peak TCSPC-channel) You can also add additional information... ts can be set (e.g. 0 as minimum lifetime) in the TCSPC fitting panel * the fast lifetime in an ideal s
- how_to_work_with_the_instrument_response_function_irf
- 57fcfa684daef86c23]].\\ 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; {{ howto:tcspc_decay_gui.png?400 |}} Response: you see this kind of curve: {{ howt... as these decays contain the noise of the complete TCSPC-window, the end of the fitting curve might show n... ype of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser
- visualizing_dynamics_with_the_multi_frame_flim_analysis
- detected, is randomly spread over the [[glossary:TCSPC]] window, the mean photon arrival time of the dar... is usually very long (up to 1/2 of the [[glossary:TCSPC]] window), which makes the color scale loaded by ... pplied to the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\ - **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green
- calculate_fccs_trace_with_the_grouped_fcs_script
- r 1), move the red bar to the right to expand the TCSPC selection over the whole TCSPC window for that channel. The complete TCSPC window should be highlighted in green. {{ calcul
- flim-fret_calculation_for_single_exponential_donors
- onential decay kinetics. - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1)... struction is deducted from the rising edge of the TCSPC histogram. On the left of this graph, the fitting
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
- nd" as active filter, and move the red bar in the TCSPC window to the right to highlight the complete TCSPC window in green. {{ using_the_flcs_script_for_spe