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Fulltext results:
- intensity_time_trace_analysis @howto
- Plotting options. - **Center/right:** Intensity time trace. As no time traces have been calculated yet, this area is sti... space, select the file ''GUVs.ptu'' and apply the time trace script to that file. When the intensity time trace window opens, click "Calculate" using a bin... of 1 ms as set by default. {{ using_the_intensity_time_trace_script_Image_12.png?600 }} **Note:** When the intensity time trace of an image is displayed, the scanning resu
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- e mean product of the fluorescence intensity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence intensity squa... -Weighted FCS and 2D FLCS:Advanced Application of Time-Tagged TCSPC" \\ Gregor and Enderlein,2007 "Time Resolved methods in biophysics. 3. Fluorescence L... orrelation Spectroscopy" \\ Bohmer et al., 2002, "Time-resolved fluorescence correlation spectroscopy" ... of fluorescence correlation spectroscopy by using time-correlated single photon counting. \\ Triffo et a
- burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
- = 4.2 Physical interpretation ==== The diffusion time $t_D$ is not the mean residence time of an individual molecule. Instead, it is a corre... entally different observables. The FCS diffusion time $t_D$ is a correlation-derived geometry time scale governed by quadratic spatial weighting of ... relation. Flow introduces an additional transport time scale that leaves the FCS diffusion time unchanged when modeled explicitly, while directly... ely confined to slow non-stationarity. Diffusion time and burst duration should therefore be regarded a
- software
- nce-Tools/tttrlib|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files. *[[https://github.com/y... tware * [[https://timetag.github.io|Extensible Time-tag Analyzer]]. ETA, the extensible time tag analyzer, is an event driven programming lang... ab/readPTU]] readPTU: a Python Library to Analyse Time Tagged Time Resolved Data [[http://qpl.eps.hw.ac.uk/]], [[htt... w level, high performance API to read and process time-tagged-time resolved (TTTR) data * [[https://github.
- t3-mode @glossary
- vent and the last sync event, and - the arrival time of the event pair on the overall experiment time scale (the time tag). The latter was originally obtained from a... his furthermore allows to reconstruct the arrival time of the photon with respect to the overall experiment time. T3 Mode measurements always implicitly provide ... ssing of the data stream a theoretically infinite time span can be recovered. The 12 bits for the start-stop time difference cover a time span of 4096*R where R is... ts with much higher rate can be recorded for some time. Only if the average count rate over a long period of time exceeds the readout speed of the PC, a FIFO overr
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- ** Fitting and analysis options\\ - **Center:** Time traces. As the time traces are not yet calculated, this graph is stil... , calculate the FCS curve and check the diffusion time to see the average residence time of the sample in the laser focus. {{ calculate_r... tion) conditions, which is visualized in the "Set Time Gate" area. In this example first a 530 nm pulse ... and acceptor channel were recorded with a slight time delay, therefore two peaks can be seen in this ar
- t2-mode @glossary
- 4 bits for the channel number and 28 bits for the time-tag. Routing is not supported. If the time tag overflows, a special overflow marker record i... ssing of the data stream a theoretically infinite time span can be recovered at full resolution. [[Dead time]]s exist only within each channel (95 ns typ.) bu... ts with much higher rate can be recorded for some time. Only if the average count rate over a long period of time exceeds the readout speed of the PC, a FIFO overr... should be able to store 28 bit = 228 = 268435456 time bin data before need to "wrap around". However, the real wrap around time as shown in the demo code is only 210698240. This
- some_origins_of_multiexponetial_decays_for_single_dyes
- arizers at magic angle, the rotation correlation time shows up as a second exponential in the decay (th... s in fact the product of the rotation correlation time and the fluorescence lifetime, divided by their s... riginal fluorescence lifetime, the observed decay time measured with a TCSPC spectrometer may be single-... o $\tau_A$ and $\tau_B$.// In this case the decay time measured in the spectral region of A would be sin
- create_time_gated_image
- :flim-image_time-gate_selection.png|}} * Now a time gat can be defined by moving the red bars. Alternatively a time can be entered in the field above. Here we select... mage. {{:gatedimageexport.png|}} * If a second time-gated image should be selected, either just select a new time gate as described above and repeat these steps. * If you want to simply create a second time-gated image with the complementary photon selecti
- calculate_ratiometric_single_pair_fret_distributions @howto
- d analysis options.\\ - **Upper center/right:** Time traces. As the time traces are not calculated yet, this graph is stil... , calculate the FCS curve and check the diffusion time to see the average residence time of the sample in the laser focus. {{ calculate_r... and acceptor channel were recorded with a slight time delay, therefore two peaks can be seen in the gre... IE-FRET Script]]). **Response: **\\ * Three time traces are calculated and displayed in the window
- avoid_pile_up_effect_in_flim_measurements @howto
- orrect average count rate.)) * At a pixel dwell time of 10µs each pixel is displayed as one point in the time trace. At a pixel dwell time of 1 µs the average of 10 pixels are displayed as one point in the time trace. * During the Test FLIM measurement the laser intensity is set th... s present. Procedure: * Calculate the exposure time per pixel: Multiply the number of frames with the pixel dwell time * Determine the number of photons via the data ... e the number of photons per pixel by the exposure time per pixel ===== Differential Count Rate ===== se
- how_to_measure_the_instrument_response_function_irf @howto
- ved. Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose. =... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- page.\\ - **Upper center/right:** the intensity time trace. The display option can be changed using th... ing is not correlated with the laser pulse on the time - range of interest, photons caused by detector a... cs at a timescale short or equal to the diffusion time through the confocal volume. Examples for these d... It can be clearly seen that at short correlation time ranges, the curve does not fit the data well. Thi
- pile-up_effect @glossary
- detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the next photon to detect. The dead time for TCSPC devices used for the measurement of flu... e every 25 ns a laser pulse. Considering the dead time we can detect only one photon after one laser sho... lost at hight photon count rates due to the dead time of the TCSPC devices. There are two effects:
- applications
- ments]] [[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time|Burst Duration and FCS Diffusion Time]] ==== Single Molecule Detection (SMD) ==== [[... tions]] [[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time|Burst Duration and FCS Diffusion Time]] ==== Anisotropy ==== [[howto:using_the_aniso