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Fulltext results:
- lifetime_fitting_using_the_flim_analysis @howto
- hows step-by-step, how the FLIM analysis of SymPhoTime 64 can be used to perform a lifetime fit and how to extract and read the results. In d... ile the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... which makes the color scale loaded by default sometimes unsuited for displaying the lifetime contrast in the actual sample. In this case, the ... s on the right of the plot.\\ **Lower left:** Lifetime fitting parameters. Here lifetime fitting models can be defined. Fitting is then ap
- flim_fret_calculation_for_multi_exponential_donors @howto
- portant to use the amplitude weighted average lifetime. While the intensity weighted average lifetime corresponds to the real average arrival time of the photons and is therefore also close to the Fast lifetime in case of low background noise, the amplitude weighted average lifetime reflects the quenching of the donor due to the FR... ile the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... which makes the color scale loaded by default sometimes unsuited for displaying the lifetime contrast in the actual sample. In this case, the
- software
- orescent Imaging Analysis Software * [[https://timetag.github.io|Extensible Time-tag Analyzer]]. ETA, the extensible time tag analyzer, is an event driven programming lang... unting (TCSPC) data sets, namely fluorescence lifetime and time-resolve anisotropy decays. * [[https://github.c... ling TCSPC hardware and generate fluorescence lifetime images in real time. The main part of the source code is free for aca... ab/readPTU]] readPTU: a Python Library to Analyse Time Tagged Time Resolved Data [[http://qpl.eps.hw.ac.uk/]], [[htt
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- tains now the fitted, intensity weighted mean lifetime, instead of the "fast Lifetime", based on the photon arrival time only. The Histogram on the right of the image con... d distribution of the intensity-weighted mean lifetime. The distributions for lifetime component 1 and lifetime component 2 appear as bars, as these parameters h... the result image after the fit with the fast lifetime image above, you will see that the lifetime distribution of the lifetime within a single bead is much more uniform, as eff... orrected for.\\ * If you want to export the lifetime distribution, open the context menu via right mou
- intensity_time_trace_analysis @howto
- {{tag> tutorial howto time-trace analysis SymPhoTime}} ~~NOTOC~~ ======Intensity Time Trace Analysis====== ===== Summary =====... start the script ==== * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace v... ript_Image_4.png }} **Response:** The "Intensity Time Trace" script is applied to the file ''Cy5_immo_Lifetime_Trace.ptu''. Thereby, a new Window opens: {{ usin... Plotting options. - **Center/right:** Intensity time trace. As no time traces have been calculated yet, this area is sti
- lifetime-fitting_using_the_flim_script @howto
- {{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}} ====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ====== ===== Introduction ===== This tutorial shows step-by-step, how the FLIM script of SymPhoTime 64 (V2.5) can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read t... ile the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- {{tag>MicroTime LSM Theory SymPhoTime FCS Correlation}} ====== Introduction to Fluorescence Correlation Spectroscopy ======... e mean product of the fluorescence intensity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence intensity squa... interaction studies in cell membranes" * **Lifetime-filtered FCS** \\ Ishii et al., 2015, "Lifetime-Weighted FCS and 2D FLCS:Advanced Application of Time-Tagged TCSPC" \\ Gregor and Enderlein,2007 "Time Resolved methods in biophysics. 3. Fluorescence L
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- {{tag>howto analysis lifetime tutorial SymPhoTime}} ~~NOTOC~~ ====== SymPhoTime Lifetime Fitting ====== ===== Summary ===== This tutorial shows step-by-ste... and start the script * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace v... SPC histogram it can be clearly seen that the lifetime of this dye is significantly shorter than the lifetime of ATTO655. {{ lifetime_fitting_using_the_tcpsc
- flim-fret_calculation_for_single_exponential_donors @howto
- This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of [[products:SymPhoTime64|SymPhoTime 64]] can be used to calculate pixel-by-pixel the ... ce of FRET (τ<sub>D</sub>), while the shorter lifetime (τ<sub>DA</sub>) is varied. This lifetime is considered to be the donor lifetime with a FRET acceptor present. The FRET efficiency... ws a single peak, which is in contrast to the lifetime variations in the FLIM image. The explanation can... ram plots the amplitude fraction of the donor lifetime in presence of FRET. As the amplitude of a donor
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- ile the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... which makes the color scale loaded by default sometimes unsuited for displaying the lifetime contrast in the actual sample. In this case, the ... \\ **4: Decay Fitting Parameters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(s) on the lower center ([[glossary:TCSPC]] ... cale by typing the limit values for the "Fast Lifetime [ns]" and "Events[cnts]". Additionally set the "Lifetime Histogram" range on the right hand.\\ {{ howto:f
- roi_fitting_using_the_flim_script @howto
- [[glossary:flim|FLIM]] script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of intere... start the script ==== * Start [[products:SymPhoTime64|SymPhoTime 64]] software. * Open the "Samples" workspace v... ile the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... which makes the color scale loaded by default sometimes unsuited for displaying the lifetime contrast in the actual sample. In this case, the
- update @howto
- ile the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... which makes the color scale loaded by default sometimes unsuited for displaying the lifetime contrast in the actual sample. In this case, the ... \\ **4: Decay Fitting Parameters**\\ Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(s) on the lower center ([[glossary:TCSPC]] ... cale by typing the limit values for the "Fast Lifetime [ns]" and "Events[cnts]". Additionally set the "Lifetime Histogram" range on the right hand. * Tick the
- symphotime_tips_and_tricks @howto
- , which calculates the mean of the photon arrival times, you will notice that the lifetime values have partially negative values: {{fastfli... time) in the TCSPC fitting panel * the fast lifetime in an ideal system should equal the intensity weighted average lifetime [τ av. int.]. Usually, fitted lifetimes tend to be slightly shorter, as the background photons are excl... images obtained with PIE-excitation, the fast lifetimes are usually meaningless, as in this case the pho
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- re (i.e. the piezo scanner in the [[products:MicroTime#MicroTime 200|MicroTime200]]), the image dimensions are automatically cor... ded, a procedure according to the tutorial: [[Lifetime fitting using the FLIM Analysis]] must be applied... 17 images have to be analyzed, which can be quite time consuming. Therefore, it can be helpful to limit
- burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
- = 4.2 Physical interpretation ==== The diffusion time $t_D$ is not the mean residence time of an individual molecule. Instead, it is a corre... entally different observables. The FCS diffusion time $t_D$ is a correlation-derived geometry time scale governed by quadratic spatial weighting of ... relation. Flow introduces an additional transport time scale that leaves the FCS diffusion time unchanged when modeled explicitly, while directly... ely confined to slow non-stationarity. Diffusion time and burst duration should therefore be regarded a