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Fulltext results:
- intensity_time_trace_analysis @howto
- Plotting options. - **Center/right:** Intensity time trace. As no time traces have been calculated yet, this area is sti... space, select the file ''GUVs.ptu'' and apply the time trace script to that file. When the intensity time trace window opens, click "Calculate" using a bin... of 1 ms as set by default. {{ using_the_intensity_time_trace_script_Image_12.png?600 }} **Note:** When the intensity time trace of an image is displayed, the scanning resu
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- e mean product of the fluorescence intensity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence intensity squa... -Weighted FCS and 2D FLCS:Advanced Application of Time-Tagged TCSPC" \\ Gregor and Enderlein,2007 "Time Resolved methods in biophysics. 3. Fluorescence L... orrelation Spectroscopy" \\ Bohmer et al., 2002, "Time-resolved fluorescence correlation spectroscopy" ... of fluorescence correlation spectroscopy by using time-correlated single photon counting. \\ Triffo et a
- software
- orescent Imaging Analysis Software * [[https://timetag.github.io|Extensible Time-tag Analyzer]]. ETA, the extensible time tag analyzer, is an event driven programming lang... ab/readPTU]] readPTU: a Python Library to Analyse Time Tagged Time Resolved Data [[http://qpl.eps.hw.ac.uk/]], [[htt... w level, high performance API to read and process time-tagged-time resolved (TTTR) data * [[https://github.... PCK, PCO, PFS, PUS, and PQRES. PTU files contain time correlated single photon counting (TCSPC) measure
- burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
- = 4.2 Physical interpretation ==== The diffusion time $t_D$ is not the mean residence time of an individual molecule. Instead, it is a corre... entally different observables. The FCS diffusion time $t_D$ is a correlation-derived geometry time scale governed by quadratic spatial weighting of ... relation. Flow introduces an additional transport time scale that leaves the FCS diffusion time unchanged when modeled explicitly, while directly... ely confined to slow non-stationarity. Diffusion time and burst duration should therefore be regarded a
- t3-mode @glossary
- vent and the last sync event, and - the arrival time of the event pair on the overall experiment time scale (the time tag). The latter was originally obtained from a... his furthermore allows to reconstruct the arrival time of the photon with respect to the overall experiment time. T3 Mode measurements always implicitly provide ... ssing of the data stream a theoretically infinite time span can be recovered. The 12 bits for the start-stop time difference cover a time span of 4096*R where R is... resolution R but of course only starting from lag times larger than the [[dead time]]. The 32 bit event records are queued in a [[FI
- t2-mode @glossary
- 4 bits for the channel number and 28 bits for the time-tag. Routing is not supported. If the time tag overflows, a special overflow marker record i... ssing of the data stream a theoretically infinite time span can be recovered at full resolution. [[Dead time]]s exist only within each channel (95 ns typ.) bu... s correlations can be calculated down to zero lag time. This allows powerful new application such as [[FCS]] with lag times from picoseconds to hours. Autocorrelations can ... l resolution but of course only starting from lag times larger than the [[dead time]]. The 32 bit event records are queued in a [[FI
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- ** Fitting and analysis options\\ - **Center:** Time traces. As the time traces are not yet calculated, this graph is stil... , calculate the FCS curve and check the diffusion time to see the average residence time of the sample in the laser focus. {{ calculate_r... tion) conditions, which is visualized in the "Set Time Gate" area. In this example first a 530 nm pulse ... and acceptor channel were recorded with a slight time delay, therefore two peaks can be seen in this ar
- some_origins_of_multiexponetial_decays_for_single_dyes
- arizers at magic angle, the rotation correlation time shows up as a second exponential in the decay (th... s in fact the product of the rotation correlation time and the fluorescence lifetime, divided by their s... riginal fluorescence lifetime, the observed decay time measured with a TCSPC spectrometer may be single-... o $\tau_A$ and $\tau_B$.// In this case the decay time measured in the spectral region of A would be sin
- create_time_gated_image
- :flim-image_time-gate_selection.png|}} * Now a time gat can be defined by moving the red bars. Alternatively a time can be entered in the field above. Here we select... mage. {{:gatedimageexport.png|}} * If a second time-gated image should be selected, either just select a new time gate as described above and repeat these steps. * If you want to simply create a second time-gated image with the complementary photon selecti
- calculate_ratiometric_single_pair_fret_distributions @howto
- d analysis options.\\ - **Upper center/right:** Time traces. As the time traces are not calculated yet, this graph is stil... , calculate the FCS curve and check the diffusion time to see the average residence time of the sample in the laser focus. {{ calculate_r... and acceptor channel were recorded with a slight time delay, therefore two peaks can be seen in the gre... IE-FRET Script]]). **Response: **\\ * Three time traces are calculated and displayed in the window
- avoid_pile_up_effect_in_flim_measurements @howto
- orrect average count rate.)) * At a pixel dwell time of 10µs each pixel is displayed as one point in the time trace. At a pixel dwell time of 1 µs the average of 10 pixels are displayed as one point in the time trace. * During the Test FLIM measurement the laser intensity is set th... s present. Procedure: * Calculate the exposure time per pixel: Multiply the number of frames with the pixel dwell time * Determine the number of photons via the data ... e the number of photons per pixel by the exposure time per pixel ===== Differential Count Rate ===== se
- tttr @glossary
- ====== TTTR ====== TTTR stands for Time-Tagged Time-Resolved. The basic concept of this measurement mode is to reco... hoton of a [[TCSPC]] experiment. Both the arrival time as well as the TCSPC timing information (i.e. the time from the preceding excitation pulse or to the fol... stop experiments) are recorded. The [[Products:TimeHarp 200]] (also the [[Products:TimeHarp 100]]), the [[Products:PicoHarp 300]] [[Produ... The traditional recording as with the [[Products:TimeHarp 200]] / [[Products:TimeHarp 100]] is called [[T3-mode]]. In [[T2-mode]] b
- how_to_work_with_the_instrument_response_function_irf @howto
- ths of all contributions. The observed decay in a time domain measurement is a convolution of the 'real'... see this kind of curve: {{ howto:irf_decay_before_timegate.png?800 |}} (Before selecting a detection cha... pdated.\\ 4. Select the decay range by Setting a `Time Gate` (move the red bars) to limit the decay the ... **Note:** * The green area is used for the timegate. * In order to define the time range bette
- how_to_measure_the_instrument_response_function_irf @howto
- ved. Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose. =... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
- using_the_antibunching_script @howto
- mber of simultaneously emitting molecules, a dead time free setup has to be employed for that time range. The SymPhoTime 64 script analyses data rec... g the TCSPC units PicoHarp 300, HydraHarp 400 and TimeHarp 260 PICO dual channel board, respectively.\\ ... citation may be used, but usually the measurement time with cw light is significantly larger. Therefore,... elp page.\\ - **Upper center/right:** Intensity time trace. The display can be changed using the "Trac