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- t3-mode
- vent and the last sync event, and - the arrival time of the event pair on the overall experiment time scale (the time tag). The latter was originally obtained from a... his furthermore allows to reconstruct the arrival time of the photon with respect to the overall experiment time. T3 Mode measurements always implicitly provide ... ssing of the data stream a theoretically infinite time span can be recovered. The 12 bits for the start-stop time difference cover a time span of 4096*R where R is... ts with much higher rate can be recorded for some time. Only if the average count rate over a long period of time exceeds the readout speed of the PC, a FIFO overr
- t2-mode
- 4 bits for the channel number and 28 bits for the time-tag. Routing is not supported. If the time tag overflows, a special overflow marker record i... ssing of the data stream a theoretically infinite time span can be recovered at full resolution. [[Dead time]]s exist only within each channel (95 ns typ.) bu... ts with much higher rate can be recorded for some time. Only if the average count rate over a long period of time exceeds the readout speed of the PC, a FIFO overr... should be able to store 28 bit = 228 = 268435456 time bin data before need to "wrap around". However, the real wrap around time as shown in the demo code is only 210698240. This
- pile-up_effect
- detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the next photon to detect. The dead time for TCSPC devices used for the measurement of flu... e every 25 ns a laser pulse. Considering the dead time we can detect only one photon after one laser sho... lost at hight photon count rates due to the dead time of the TCSPC devices. There are two effects:
- differential_count_rate
- {{tag> pile-up dead_time IRF differential_countrate}} ====== Differential Count Rate ====== Ideally, a... y (probability of encountering a photon) during a time interval when a detected signal is really present... the detector bunched, as flashes. These are short time intervals with huge photon density (rate), separa... rate" is a result of **sparse sampling with dead time**. The sampled signal features much higher photon
- dead_time
- ====== Dead Time ====== In TCSPC the term dead time refers to the time the TCSPC system needs armed again after detecting an event. During the dead time the TCSPC system is blind. If for example two photons are detected with the dead time of the TCSPC device the second photon will be los
- tttr
- ====== TTTR ====== TTTR stands for Time-Tagged Time-Resolved. The basic concept of this measurement mode is to reco... hoton of a [[TCSPC]] experiment. Both the arrival time as well as the TCSPC timing information (i.e. the time from the preceding excitation pulse or to the fol... t' and 'Stop') as two separate TTTR traces with a time resolution of 4ps, a new TTTR mode was introduced
- mcs
- surement are sorted by means of their macroscopic time into equally spaced time bins. What immediately results is a time trace of the fluorescence intensity. In a subsequ... form [[TCSPC]] histograms, which in turn can be used for lifetime analysis, time-gated analysis etc.
- deconvolution
- == Deconvolution ====== Deconvolution is used in time domain data analysis for removal of broadening ef... nvolution, the best way of doing it (at least for time domain data) would be via Fourier transform. Four... or deconvolution-like) techniques. What holds for time domain data, also holds for imaging: Deconvolutio
- tcspc
- ed into histogram channels that correspond to the time that elapsed since the last excitation pulse. It is a time domain method for investigating time resolved fluorescence.
- bootstrap
- d fitted, the bootstrap method seems to be rather time consuming. On the other hand all parameter errors... one go"; also the simulation process is not very time consuming. The bootstrap method makes far less as
- convolution
- as $C(t)=\int_{-\infty}^t F(t-t')~G(t')~dt'$ In time resolved fluorescence it describes the effects of... Dec(t-t')~IRF(t')~dt'$ where $Dec(t)$ is the unconvolved ('real') decay starting at a given time $t=0$
- pie
- sources). The laser pulses are separated on a ns time scale to allow simultaneous recording of the time response of a sample molecule due to each individ
- bifl
- thods afterwards. Usually a burst is defined as a time interval for which the intensity of a time trace or [[MCS]] measurement is above a chosen th
- fast_lifetime
- the barycentre of the (pseudo–)pixel's decay. The time span from the barycentre of the IRF to the baryce... statistics. If an [[IRF]] is not available, the "time zero" $t_\theta$ has to be estimated differently,
- least_squares
- he model describes the data correctly. *The experimental noise along the time axis is negligible.