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Fulltext results:
- flim_fret_calculation_for_multi_exponential_donors @howto
- pplication note [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]]. Th... The window contains five different regions:\\ **Upper left:** Imaging analysis options.\\ **Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
- avoid_pile_up_effect_in_flim_measurements @howto
- easurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile-up effect]] the measured count rate should not exceed a certain li... or most FLIM measurements this relative weak pile up effect is acceptable. If even higher precision ... ate of the laser. ===== Measurement of the Pile Up Limit ===== * For setting the right laser int
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- es through the detection volume of a confocal set-up. Since then a series of studies have been perfor... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fl... in FCS studies. Assume that we use the same set-up to measure the diffusion of three globular fluo... regarding: * [[http://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficient
- lifetime-fitting_using_the_flim_script @howto
- me and contains e.g. background photons.\\ * An updated lifetime frequency histogram on the upper right. The individual lifetime components are
- separation_of_2_species_with @howto
- s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ separation_of_2_species_with_Image_8.png?600 ... ut the meaning of the different parameters. - **Upper center:** TCSPC histogram of the imported patt... n yet, this graph doesn't contain any data. - **Upper right:** TCPSC curve of the measured data. Low
- pile-up_effect @glossary
- tion pulse is negligible. * [[howto:avoid_pile_up_effect_in_flim_measurements|How to Avoid the Pile-up Effect in FLIM Measurements]] ===== Example of pile up effect ===== We have performed an example measur... ional short component lifetime could still not be detected {{ :glossary:pile-up_example.png?600 |}}
- configuring_symphotime64_after_installation @support
- ce driver and press on "Connect". * You can set up the Laser Heads by pressing "Setup Heads" and a new window will pop up: {{ :support:laser_settings_setup_head.png?300 |... Do not forget to press "Save Head" after setting up each Laser Head" </WRAP> ====3. For LSM upgrade kits==== Go to the SymPhoTIme Manual page 6 under "... ile to a folder so that you can have it as a back-up in case the settings are lost. * Now you are re
- symphotime_tips_and_tricks @howto
- post-acquisition analysis is performed - speeds up the processing for additional analysis. - This ... times) * Frames (especially important for LSM upgrade kits where in general several frames are rec... with the MT200 this is done automatically, in LSM upgrade systems, which are not remotely controlled b... d "Pattern Matching" the feature is made availble upon clicking on the **"Resolution estimation"** tab
- lifetime_fitting_using_the_flim_analysis @howto
- e and contains e.g. background photons. * An updated lifetime frequency histogram on the upper right. The fixed individual lifetime component
- microtime @products
- .com|PicoQuant GmbH]] ===== MicroTime 100 ===== Upright Time-resolved Fluorescence Microscope * Complete system with laser combining unit, upright microscope body and detection unit * Pulse... 75 nm to 810 nm * Multiple detector options for up to four detection channels * XY-scanning piezo ... imaging * Wide range scanner with a scan range up to several cm See the picoquant.com webpage for
- calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
- d from calculating the FCS trace by adjusting the upper vertical red limit bars in the small upper trace.\\ - **Lower center/right:** FCS trace... }} **Response:** The FCS Calibration window pops up. {{ calibrate_the_confocal_volume_for_fcs_using_t... ript_Image_21.png }} **Response:** A window pops up asking to confirm the calibration values. * Cli... rmed whenclosing the software. A message will pop up when closing SymPhoTime 64: {{ calibrate_the_con
- supported_mt200_pc_configuration_for_symphotime_32 @software
- ivers for Network, Mainboard etc. * Run windows update to fetch all the latest updates * Install the Peak CAN PCI card * Start ... pported: TimeHarp 200, all versions PicoHarp 300, up to version HydraHarp 400, up to version 1.2 * NOT SUPPORTED: TimeHarp 260, H
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
- s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ using_the_flcs_script_for_spectral_crosstalk_... ut the meaning of the different parameters. - **Upper center:** The graph for the imported patterns.... n yet, this graph doesn't contain any data. - **Upper right:** A graph containing the TCPSC curve of
- flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade @howto
- {{tag> FLIM acquisition Nikon demo video LSM_upgrade howto}} ====== FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade ====== This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Nikon A1. In FLIM-measur... of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2014 and
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}} ====== Lifetime-Fi... rdware with count-rates way beyond classical pile up limit. For the principle of rapidFLIM - recordi... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... |}} **Response:**\\ FLIM Preview image will get updated. You may need to adapt the contrast again. O